N-Ethyl-N (3-two-aminoethyl) carbonic amide (also known as EDC, EDAC, or EDCI) is a water-soluble carbon diamine, often made into hydrochloric acid form. Its ph range is usually 4.0-6.0. EDC is often used as a carboxyl activator in the preparation of amide ammonia. EDC can also be used to activate phosphoric acid groups. This carbon diamine is commonly used in the synthesis of peptides, the cross-linking of proteins and nucleic acids, and the preparation of immune coupling compounds. EDC is often used with n-hydroxy-imide (NHS) or sulfonated-NHS to increase the efficiency of coupling reactions. In organic chemistry, EDC is often used with dmap as a esterification reagent for carboxylic acids and alcohols.